The Relationship between p38 MAPK Pathway and Adriamycin Resistance of Breast Carcinoma MCF-7/Adr Cells

Objective To investigate the relationship between p38MAPK pathway and the adriamycin resistance of breast carcinoma cells.Methods Using p38MAPK inhibitor SB203580 to analyze the effect on the cell apoptosis through FITC-Annexin-V/PI double staining.The concentration of adriamycin was detected by the flow cytometry(FCM).Results After intervening with SB203580 for 24 hours and 48 hours the apoptosis efficiency was increased remarkably.They were much higher in 24h group(25.36±1.17)% and 48h group(38.21±1.25)%) than in control group(0.65±0.21)% and DMSO group(0.81±0.16)%(P0.01).And the cell apoptosis efficiency increased in a time-dependent manner(P0.05);Inhibition of p38MAPK pathway could increase cellular adriamycin accumulation: It was higher in 24h group(32.45±2.36) and 48h group(41.66±3.12) than in control group(14.17±1.45) and DMSO group(16.28±0.63)(P0.01).Conclusion There has a closely relationship between p38MAPK pathway and the adriamycin resistance of breast carcinoma cells.