Influences of Jianpi Shugan Fang on ADP and TNF-α in rats with non-alcoholic steatohepatitis

Objective To observe the curative effect of Jianpi Shugan Fang on non-alcoholic steatohepatitis(NASH) and its influences on the expressions of adiponectin(ADP) and tumor necrosis factor-α(TNF-α).Methods SD male rats(n=40) were randomly divided into the blank group,model group,Jianpi Shugan Fang group and Yishanfu Capsules group.The model was established with high fat forage for 8 weeks and then with normal forage after successfully modeling.At the same time,the Jianpi Shugan Fang group was intragastrically given extractum of Jianpi Shugan Fang and Yishanfu Capsules group,suspension of Yishanfu Capsules,and the model group and blank group were given deionized water for 4 weeks.The pathological changes of liver tissue were observed,and the levels of liver function,blood fat and hepatic lipid,and expression of ADP and TNF-α were detected.Results In Jianpi Shugan Fang group,the body weight,hepatic index,hepatic steatosis and inflammatory lesion,and levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST) and hepatic fat content were all lower significantly than those in the model group(P0.01),but not different compared with Yishanfu Capsules group(P0.05).The expression of TNF-α mRNA decreased and that of ADP mRNA increased,and they were statistically different compared with the model group(P0.01).Conclusion Jianpi Shugan Fang has a good curative effect in NASH rats,and it can prevent and treat NASH through inhibiting inflammatory reaction,and protecting liver against inflammation,necrosis and apoptosis.