Experiment Study of Chuangyugao on bFGF Content in Granulation Tissue in Rats

Objective: To detect the content of bFGF in rat skin ulcer wound granulation tissue and to explore the mechanism of Chuangyugao promoting ulcer healing. Methods: 60 SD rats were randomly divided into Group A( Chuangyugao),group B( MEBO)and group C( normal saline group),n = 20. The full- thickness excision injury models of those rats were made,changing the dressing every 12 hours. The granulation tissue of wounds was taken out after modeling for 3,7,14,21 d,detecting the content of bFGF in wounds with ELISA assay. Results: The expression of bFGF in the granulation tissue in Chuangyugao group was much higher than that of other two groups. There was significant difference through pair wise comparison. And on the 7th day,there was no difference between Chuangyugao group and MEBO group in the expression of bFGF in the granulation tissue,however,compared with the normal saline group,it was significantly lower and there were significant differences. Conclusion: Chuangyugao through the promotion of basic fibroblast proliferation and the growth of granulation tissue can shorten the healing time and improve the rate of wound healing.