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2009•Southern HorticultureRequires access

The technique of tissue culture for Musa spp.Longyajiao

Yang Hui-wen

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Abstract

Adventitious buds were induced from shoot-tip explants of Musa spp Longyajiao in MS+6-BA5.0 mg/L+AD 20 mg/L.The multiplication medium with MS+BA 4 mg/L+NAA 0.1 mg/L was better for the buds growth and the average multiplication rate was over 2.6 times.Plantlets were cultured on 1/2 MS+NAA 0.5 mg/L+AC 1 g/L,for rooting culture,after 7 days the roots of the plantlets were developed.The regenerated plantlets were transplanted to soil,and the survival rat of transplanting reached 94%.The plants grew well in the field.

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What this paper is about

Adventitious buds were induced from shoot-tip explants of Musa spp Longyajiao in MS+6-BA5.0 mg/L+AD 20 mg/L.The multiplication medium with MS+BA 4 mg/L+NAA 0.1 mg/L was better for the buds growth and the average multiplication rate was over 2.6 times.Plantlets were cultured on 1/2 MS+NAA 0.5 mg/L+AC 1 g/L,for rooting culture,after 7 days the roots of the plantlets were developed.The regenerated plantlets were transplanted to soil,and the survival rat of transplanting reached 94%.The plants grew well in the field.

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Available abstract

Adventitious buds were induced from shoot-tip explants of Musa spp Longyajiao in MS+6-BA5.0 mg/L+AD 20 mg/L.The multiplication medium with MS+BA 4 mg/L+NAA 0.1 mg/L was better for the buds growth and the average multiplication rate was over 2.6 times.Plantlets were cultured on 1/2 MS+NAA 0.5 mg/L+AC 1 g/L,for rooting culture,after 7 days the roots of the plantlets were developed.The regenerated plantlets were transplanted to soil,and the survival rat of transplanting reached 94%.The plants grew well in the field.

Key concepts: Transplanting, Explant culture, Shoot, Tissue culture, Biology, Botany, Murashige and Skoog medium, Musaceae

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