Effect of Bazhen decoction on the spleen T lymphocytes and serum levels of cytokines in tumor-bearing mice after chemotherapy

Objective: To discover the effect of Bazhen decoction on the proliferation,subpopulations of spleen T lymphocytes and serum levels of cytokines in tumor-bearing mice after chemotherapy. Methods: Thirty tumor-bearing model of mice were established. The mice were divided randomly into three groups: Model group,CTX group,Bazhen decoction + CTX group. Then taken ten healthy mice for normal control group. Mice in CTX group and Bazhen decoction + CTX group were given Cyclophosphamide by intraperitoneal injection for three days,mice in normal control group and model group were given normal saline for three days,then mice in Bazhen decoction + CTX group were given Bazhen decoction by gastric perfusion for ten days,mice in normal control group and model group and model group were given normal saline by gastric perfusion for ten days. The proliferation of T lymphocytes were detected by MTT,the T lymphocyte Subpopulations were detected by Flow cytometry( FCM),ELISA were used to detected the level of IFN-γand IL-2 in the blood serum. Results: After treatment with Bazhen decoction,the proliferation of T lymphocytes of chemotherapied mice was promoted significantly( P 0. 01),and it was as same as model group( P 0. 05); the ratio of CD3+,CD3+CD4+CD8-,CD3+CD4-CD8+T lymphocytes were significantly higher than CTX group( P 0. 01),but they were lower than model group( P 0. 05);the level of IFN-γ and IL-2 in the blood serum were increased significantly( P 0. 01),and no significantly with model group( P 0. 05). Conclusion: Bazhen decoction may ameliorate the inhibition on T lymphocytes in tumor-bearing mice by chemotherapy,improve the ability of anti-tumor.