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Improvement of MTT assay by 2-chloroadenosine in activation test and cytotoxicity test of lymphocyte

Feng Zuo

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Abstract

Objective:To sutdy the effect of 2 chloroadenosine(2 ClA),which is specifically cytotoxic to macrophages,on MTT assay in activation test and cytotoxicity test of lymphocyte.Methods:Using cell culture technique,mouse splenic lymphocytes and peritoneal macrophages were cultured.Lymphocyte activation and specific cytotoxicity to tumor cells and toxicity of 2 ClA to macrophages were measured by MTT assay in the presence or absence of 2 ClA.Results:2 ClA had a strong cytotoxic effect on macrophages.When the activation test and cytotoxicity test of lymphocyte were measured by MTT assay,the optical density values of 2 ClA group was lower than that of control group,and statistic analysis showed P0 05.Conclusion:With the specific cytotoxicity to macrophages,2 ClA can exclude the influence of macrophages to MTT assay in activation test and cytotoxicity test of lymphocyte,and more reliable results will be obtained.

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Objective:To sutdy the effect of 2 chloroadenosine(2 ClA),which is specifically cytotoxic to macrophages,on MTT assay in activation test and cytotoxicity test of lymphocyte.Methods:Using cell culture technique,mouse splenic lymphocytes and peritoneal macrophages were cultured.Lymphocyte activation and specific cytotoxicity to tumor cells and toxicity of 2 ClA to macrophages were measured by MTT assay in the presence or absence of 2 ClA.Results:2 ClA had a strong cytotoxic effect on macrophages.When the activation test and cytotoxicity test of lymphocyte were measured by MTT assay,the optical density values of 2 ClA group was lower than that of control group,and statistic analysis showed P0 05.Conclusion:With the specific cytotoxicity to macrophages,2 ClA can exclude the influence of macrophages to MTT assay in activation test and cytotoxicity test of lymphocyte,and more reliable results will be obtained.

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Available abstract

Objective:To sutdy the effect of 2 chloroadenosine(2 ClA),which is specifically cytotoxic to macrophages,on MTT assay in activation test and cytotoxicity test of lymphocyte.Methods:Using cell culture technique,mouse splenic lymphocytes and peritoneal macrophages were cultured.Lymphocyte activation and specific cytotoxicity to tumor cells and toxicity of 2 ClA to macrophages were measured by MTT assay in the presence or absence of 2 ClA.Results:2 ClA had a strong cytotoxic effect on macrophages.When the activation test and cytotoxicity test of lymphocyte were measured by MTT assay,the optical density values of 2 ClA group was lower than that of control group,and statistic analysis showed P0 05.Conclusion:With the specific cytotoxicity to macrophages,2 ClA can exclude the influence of macrophages to MTT assay in activation test and cytotoxicity test of lymphocyte,and more reliable results will be obtained.

Key concepts: Cytotoxicity, MTT assay, Lymphocyte, Cytotoxic T cell, Molecular biology, Chemistry, In vitro, Immunology

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Improvement of MTT assay by 2-chloroadenosine in activation test and cytotoxicity test of lymphocyte — Research Paper | ScholarLens