Study on apoptosis of hepatocarcinoma cell line HuH-7 induced by the wild type p53 gene

Objective To investigate the effect of apoptosis of tumor cells induced by the wild type p53(wt-p53) gene,human hepatocarcinoma cell line HuH-7 was transfected with wt-p53 gene in our study.Methods While human hepatocarcinoma cell line HuH-7 was transfected with wt-p53 gene by Lipofectamine,the apoptosis in the cells was evaluated by flow cytometry after the treatment for 96 hours.Results 2.8% in the untreated group,4.1% in transfected with pUHD 10-3 group,39.6% in tranfected with pUHD 10-3-p53 group.Conclusion Apoptosis in human hepatocarcinoma cell line HuH-7 could be induced by the wt-p53 gene.