2009•PubMedRequires access

[Effect of p21-targeted shRNA on curcumin-induced apoptosis of human hepatoma Huh7 cells].

Weizhang Wang, Bi-Yu Zhang, Wen-Jie Mei, Jianwen Mao, Ming Li

Abstract

In the present study, shRNA plasmid of pSi-p21 targeting p21 mRNA was constructed and the effect of p21 shRNA on curcumin-induced apoptosis of human hepatoma Huh7 cells was investigated. The effect of curcumin on the expression of p21 mRNA and protein and the silence efficiency of pSi-p21 were detected with RT-PCR and Western blotting. The effect of pSi-p21 on curcumin-induced apoptosis of Huh7 cells was evaluated with DAPI staining. The results showed that curcumin significantly upregulated p21 mRNA and protein expression, which was knocked down by pSi-p21 of Huh7 cells. DAPI staining results showed that pSi-p21 significantly decreased curcumin-induced apoptosis of Huh7 cells. The data suggested that curcumin induced apoptosis of Huh7 cells via upregulation of p21 expression.

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Key concepts: Curcumin, DAPI, Small hairpin RNA, Apoptosis, Downregulation and upregulation, Blot, Molecular biology, Chemistry