Mutation Screening of L-histidine Producing Strain by UV and LiCl

A L-histidine producing strain was mutated and screened from Corynebacterium glutamicum S6 by LiCl and UV. Results showed that the UV-induced lethality was 81.8% with the irradiating time of 30s, while the LiCl-induced lethality was 82.4% with the LiCl concentration of 1.2%. Using three mutants, D3 by LiCl, U1 by UV, and N1 multiple mutated by UV and LiCl, the L-histidine yields reached 256mg/L, 243mg/L and 251 mg/L, respectively, which were 8.5%, 10.5% , and 13.6% respectively higher than that by the original strain S6.