2013•Science and Technology of Food IndustryRequires access
Breeding of high β-galactosidase producing Aspergillus Oryzae by mutation with UV and LiCl
Weibing Zhang
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Abstract
In order to breed high-yield β-Galactosidase producing strain,Aspergillus Oryzae(ATCC20423)was used as the original strain and mutagenized with Ultraviolet(UV)and UV+lithium chloride(LiCl),respectively.After mutagenized by UV,the β-Galactosidase activity of UV-15-20 reached 114.08U/mL,which revealed a 49.22% increase compared with the original strain.A mutant UV-LiCl-38 with high β-Galactosidase activity(121.42U/mL)was obtained by use of UV and LiCl,the β-galactosidase activity of this mutant was 58.82% higher than that by the wild-type.UV-15-20 and UV-LiCl-38 had good hereditary stability after several generations.