Effect of Ellagic Acid on Myeloma SP2/0 Cells

Objective To investigate the effect of ellagic acid extracted from gallnut on multiple myeloma SP2 /0 cell line and related mechanisms. Methods Multiple myeloma SP2 /0 cell line was treated for 48 h with different concentrations of ellagic acid in vitro. Cell morphology,proliferation,apoptosis and cell cycle were analyzed with microscope,MTT experiment and flow cytometry,respectively. Tumor cell proliferation and apoptosis-related gene expression of COX-2 were detected by Western blotting. Results Cell cycle was arrested at the G1 phase 48 h after treatment with ellagic acid,the cell in G1were(55. 21 ±3. 01)%,(64. 48 ± 0. 43)%,(75. 10 ± 2. 46)%,respectively,with significant difference as compared with control group[(34.04 ±1.74)%,P 0. 01]. Cell suppression rate(21. 18 ± 5. 92)%,(44. 58 ± 3. 43)% and(70. 15 ± 2. 90)%,respectively,in 20,40 and 60 μg·mL-1ellagic acid treatment groups. Compared with the control group,the differences were significant(P 0. 01). Cell apoptosis rate was(9. 60 ± 0. 56)%,(19. 30 ± 1. 51)% and(35. 10 ± 5. 26)%,respectively,in20,40 and 60 μg·mL-1ellagic acid treatment groups,with significant differences as compared with the control group[(3. 23 ±0. 85)%,P 0. 01]. With the increase of drug concentration,COX-2 expression was decreased. Conclusion Ellagic acid can inhibit myeloma SP2 /0 cell proliferation and promote apoptosis.