Improvement and Experience in Suture Methods for Rat Model of Focal Cerebral Ischemia

To establish a simple,stable and reliable model of focal cerebral ischemia.Methods Along with Zealonga method and modified method,the models of middle cerebral artery occlusion were created.Then after 24 h of operation,these two kinds of model were evaluated by four aspects: the neurological behavior,the cerebral infarction ratio,the relative standard deviation(RSD) of cerebral infarction ratio and the achievement ratio of model.Results All the survival rats demonstrated neurological deficit symptom after 24 h of operation.The cerebral infarction ratio between the two groups was not significant.The RSD of cerebral infarction ratio in the modified group was lower than that in the Zea-longa group while the success rate in the modified group were higher than that in the Zealonga group(χ2 = 4.37,P 0.05).Conclusion The modified method of focal cerebral ischemia model is easier to carry out and more reliable.And it is an ideal method for model of MCAO with higher success rate.