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2009•Journal of Analytical ScienceRequires access

Determination of Trace BSA by Inhibition Kinetic Spectrophotometry

LI Jian-ping

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Abstract

In this paper,the discoloration of Xylenol orange by potassium bromate in H2SO4 medium-Landolt reaction was studied.Based on this fact,a new method for the determination of trace BSA was established by inhibition kinetic spectrophotometry.Under the optimum condition,the linear range is 0.2～2.0 μg/mL,and the detection limit of the method was 0.141 mg/L.The developed method was applied to the determination of BSA with satisfactory results.

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What this paper is about

In this paper,the discoloration of Xylenol orange by potassium bromate in H2SO4 medium-Landolt reaction was studied.Based on this fact,a new method for the determination of trace BSA was established by inhibition kinetic spectrophotometry.Under the optimum condition,the linear range is 0.2～2.0 μg/mL,and the detection limit of the method was 0.141 mg/L.The developed method was applied to the determination of BSA with satisfactory results.

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Available abstract

In this paper,the discoloration of Xylenol orange by potassium bromate in H2SO4 medium-Landolt reaction was studied.Based on this fact,a new method for the determination of trace BSA was established by inhibition kinetic spectrophotometry.Under the optimum condition,the linear range is 0.2～2.0 μg/mL,and the detection limit of the method was 0.141 mg/L.The developed method was applied to the determination of BSA with satisfactory results.

Key concepts: Chemistry, Potassium bromate, Xylenol orange, Spectrophotometry, Detection limit, Linear range, Chromatography, Kinetic energy

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