Gold immunochromatography for HBsAg detection

Objective To prove the quality and performance of 3 sources of gold immunochromatography assay(GICA) strips for HBsAg detection and to provide data for various laboratiories in the selection of HBsAg assay methods.Methods A few known molar manipulative serum and 1 322 serum samples were tested with both GICA strips and ELISA.The sensitivity,specificity of the strips and pertinent experiment were assessed.Results The minimal detection limits of the 3 sources of GICA strips were 1-5ng/ml,with detection rates varying from 97.37 to 99.57%,as compared with ELISA.No false positives and pre-band phenomena were found.Conclusion GICA is fit emergency.lt is less sensitive than ELISA,and costs more.On the unusual condition,it is should be combined with ELISA.