Establishment of Polymerase Chain Reaction for Detection of Pseudorabies Virus in Pigs

Objective To establish the method of polymerase chain reaction(PCR) for detection of the pseudorabies virus(PrV) in pigs.Methods A pair of primers were designed and synthesized according to the sequence of gB gene of PrV.PCR method for PrV was established by optimizing the reaction parameters,and utilized for detection of PrV in clinical samples.Result The 263 bp product was amplified specifically by using the DNA of PrV SH strain as template.The amplified product was cloned into pMD18-T vector and sequenced.The identity of nucleotide sequence of this fragment and gB gene of genbank was 99.2%.The identical results were obtained by PCR and virus isolation of clinical samples.Conclusion The PCR method established is sensitive and specific for the rapid detection of PrV in pigs.