Isolation and Culture of Murine Primary Hepatocytes

Objective To search for an easy and cheap method for the culture of murine primary hepatocytes.Methods The murine liver was isolated by non-perfusion,digested by 0.2% IV-Collagenase so as to get the primary hepatocytes and culture them with DMEM medium in the monolayer.Results Liver cells were found under the optical microscope,showing as triangle,roundness,and so on.The cells was marshalling,and cell boundary was distinct.Cytoplasm was abundant and bright.There were one or two circular nucleuses in each cell.Conclusion This method was good for the general labs to culture the hepatocytes,and it can provide a good basic for further research.