Study of the function and clinical application in gamma irradiated apheresis platelet

Objective To study the influence of the activity of platelet by different dose of irradiation,and to evaluate the function and clinical application of gamma irradiated apheresis platelets.Methods Apheresis platelets were divided into 5 parts.Then the divided platelets were divided into control group and test group which accepted 25 Gy,30 Gy,35 Gy and 40 Gy.The lymphocytes were separated from irradiated platelets and T lymphocytes were transformed into lymphoblast stimulated by PHA,then the percent of lymphoblast was calculated by microscope after exposed to Wright strain,CD41a and CD62p of 35 Gy irradiated platelets were examined by flowcytometry.40 acute leukemia patients were divided into control group and test group,with 20 cases in each group,they were transfused apheresis platelets and irraditiated apheresis platelets,then CCI and clinical manifestation were observed.Results The percent of lymphoblast was 0 of 35 Gy irraditiated platelets and there was no significant difference of the positive rate of CD41a and CD62p before and after irradiation(P0.05).There was no significant difference of the efficiency of platelet transfusion between test group and control one(P0.05).Conclusion Appropriate dose can decrease the activity of lymphocytes but don′t impaired the activity of platelets.