Research of recombinant human endostatin combined with cisplatin on extracorporeal intervention of growth of human gastric carcinoma cell

Objective To study the influences of recombinant human endostatin(endostar)combined with cisplatin on the proliferation and apoptosis of human gastric carcinoma cell line BGC823 and SGC7901 and to explore the related mechanism.Methods Methyl thiazolyl tetrazolium(MTT)assay was used to detect the proliferative inhibition rates of different concentrations of cisplatin(single drug group) and combination of cisplatin and endostar(combination group) on BGC823 and SGC7901.Cell apoptosis was detected by flow cytometry.Changes of proliferation-related protein Ki-67 and apoptosis-related protein Bcl-2 were analyzed by Western blot.Results The inhibition rates of BGC823 and SGC7901 in the combination group were higher than those in the single drug group. Results of Flow cytometry showed that the proportion of early apoptosis was increased in turn in normal control group, single drug group and combination group. Compared with single drug group, the expression percentage of Ki-67 and Bcl-2 mRNA(protein)decreased significantly in the combination group.Conclusion Compared with the effect of cisplatin, the combination of endostar and cisplatin is proved to be much more effective in the inhibition of the proliferation and the induction of the apoptosis of cell line BGC823 and SGC7901.