Genetic Diversity of Aromatic Rice Varieties Based on Markers of Functional Genes and SSR

【Objective】Genetic diversity of aromatic rice cultivars were investigated to further provide genetic resources for breeding practice. 【Method】 Genetic diversity of 32 aromatic rice cultivars were analyzed by 22 markers of functional gene in rice and 60 SSR primers covered on 12 rice chromosomes. 【Result】A total of 188 alleles were detected, among of which 126 alleles were effective. The average number of alleles per SSR locus was 3.13 with a range from 2 to 6. The value of allelic polymorphism information content (PIC) ranged from 0.116 to 0.744, on an average of 0.467±0.175 per SSR maker. The average similarities coefficient was 0.697±0.0035 with a range from 0.51 to 0.96. UPGMA cluster analysis showed that the 32 cultivars could be classified into indica and japonica distinct classes at similarities coefficient of 0.58, which was generally coincident with pedigree analysis. 【Conclusion】SSR markers could be employed in diversity identification for aromatic varieties and aromatic rice breeding. Consequently, marker of functional gene in rice could be used for measuring genetic diversity, assigning rice to geographical distribution, and analyzing its ecotype and pedigree relationship.