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Study of extraction conditions on the carotenoid produced from Cordyceps militaris

Tingting Zhang

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Abstract

Objective: The extractions on the carotenoid produced from Cordyceps militaris were studied. Methods: The content of carotenoid was determined by UV-spectrophotometry. The optimum extraction process was selected with the orthogonal design. Result: The experimental results showed that the method combining hydrochloric acid(HCl) with heating(by boiling water) was suitable for extraction on the carotenoid produced from Cordyceps militaris. Conclusion: The optimal extraction condition was obtained as follows: HCl concentration 1mol/L, HCl addition 15mL/g(dry sample), soaking 20min and heating 4min. Acetone was the optimal extracting organic solvent. Under the above optimal conditions, the yield of carotenoid could reach 2158μg/g dry sample.

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What this paper is about

Objective: The extractions on the carotenoid produced from Cordyceps militaris were studied. Methods: The content of carotenoid was determined by UV-spectrophotometry. The optimum extraction process was selected with the orthogonal design. Result: The experimental results showed that the method combining hydrochloric acid(HCl) with heating(by boiling water) was suitable for extraction on the carotenoid produced from Cordyceps militaris. Conclusion: The optimal extraction condition was obtained as follows: HCl concentration 1mol/L, HCl addition 15mL/g(dry sample), soaking 20min and heating 4min. Acetone was the optimal extracting organic solvent. Under the above optimal conditions, the yield of carotenoid could reach 2158μg/g dry sample.

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Available abstract

Objective: The extractions on the carotenoid produced from Cordyceps militaris were studied. Methods: The content of carotenoid was determined by UV-spectrophotometry. The optimum extraction process was selected with the orthogonal design. Result: The experimental results showed that the method combining hydrochloric acid(HCl) with heating(by boiling water) was suitable for extraction on the carotenoid produced from Cordyceps militaris. Conclusion: The optimal extraction condition was obtained as follows: HCl concentration 1mol/L, HCl addition 15mL/g(dry sample), soaking 20min and heating 4min. Acetone was the optimal extracting organic solvent. Under the above optimal conditions, the yield of carotenoid could reach 2158μg/g dry sample.

Key concepts: Cordyceps militaris, Extraction (chemistry), Carotenoid, Hydrochloric acid, Acetone, Chemistry, Boiling, Yield (engineering)

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