Construction and expression of the suppression gene of human lymphocyte

ve To obtain and construct Prokaryotic expression vector of human lymphocyte suppression gene. Methods A 1493 bp cDNA fragment was amplified by RT-PCR method from total RNA of human peripheral blood lymphocyte cell activated with 160 U/mL for 12 hours. The fragment cloning into pET-11-c plasmids.The cloned insert was identified by double I digestion of the recombinant plasmid with restriction enzymes Nde T and BamH I and sequenced by Sangers-dideory-mediated chain termination. Results This cDNA fragment in-cluded 1 493 bp entire coding region. The recombinant Prokaryotic expression vector of SPN gene was constructed and the protein of SPN was expressed . Conclusion The recombinant Prokaryotic expression vector was successfully constructed. These results pave the way for researches of SPN.