Irradiation enhancement of celecoxib on human cell line of cervical cancer

Objective:To investigate the radiation enhancement effect of celecoxib on human cervical carcinoma Hela cells and its possible mechanism.Methods:MTT assay was employed to examine the growth suppression of Hela cells.The enhancement of radiosensitising effect was detected by clone formation experiment.The apoptosis rate and change in cell cycle of Hela cells treated with celecoxib or/and radiation were examined by flow cytometer.Results:The outcome of MTT assay suggested that celecoxib may produce a concentration-dependent and radiation dose-dependent inhibition of Hela cell growth.By colony-forming experiments,celecoxib showed radiosensitizing effect to a certain extent in Hela cells.The survival curve and the related parameters indicated that celecoxib could enhance the radiosensitivity of Hela cells.The value of Do and Dq of Group D+R was significantly different from group R.Flow-cytometric analysis showed cell cycle redistribution and the apoptosis rate had diffrent in group D,R and D+R,they could change cell cycle of Hela cells lines with G0/G1 phase cells increasing and S phase cells decreasing and the apoptosis rate increasing.Conclusion:Celecoxib possesses radiosensitizing effect on Hela cells in vitro,which presents a laboratory evidence for combined administration of celecoxib and irradiation to treat human cervical carcinoma.