Study on radiosensitization effect of celecoxib on human cervical cancer HeLa cells and the effect factors

Objective:To study the radiosensitization effect of celecoxib on human cervical cancer HeLa cells and the effect of administration sequence on radiosensitization.Methods:MTT method was used to detect the inhibitory concentration 50(IC 50) of Hela cells after treated with celecoxib,clone formation and single-hit multitarget model detection were used to detect the radiosensitization effect of celecoxib,cell survival rate and sensitive enhancement ratio(SER) of different administration sequences.Results:Celecoxib inhibited the proliferation of cervical cancer Hela cell strains,showing a dose-and time-dependent manner,IC 50 of Hela cells after treated with celecoxib was 44 μmol/L;after treating Hela cells with 40 μmol/L celecoxib combined with X-ray,SERs in administration prior to radiation group,administration combined with radiation group and radiation prior to administration group were 2.46,1.91 and 1.44,respectively;immunochemistry confirmed that celecoxib could down-regulate the expression of VEGF in Hela cells.Conclusion:Celecoxib has a radiosensitization effect on Hela cells,the radiosensitization effect has sequential compliance;the mechanism is related to down-regulation of VEGF expression in human cervical cancer Hela cells.