Determination of Polyphenol Substances in Yellow Rice Wine by Capillary Electrophoresis in Association with Electrochemical Detection

A high-performance capillary electrophoresis in association with electrochemical detection(CE-ED) method was developed for the determination of the content of six bioactive ingredients including epicatechin,rutin,kaempferol,hyperoside,chlorogenic acid,and quercetin in yellow rice wine.The effects of several factors such as the acidity and the concentration of running buffer,the separation voltage,the oxidation potential and the injection time on CE-ED were investigated.Under the optimum conditions including 300 μm carbon disc electrode used as detection electrode,detection potential as +950 mV(vs.SCE,and a 75 cm length capillary at a separation voltage of 16 kV in 40 mmol/L borate buffer(pH 9.0),the analytes could be well separated within 15 min.Good linear relationship was established between peak current and concentration of analytes over three orders of magnitude and their detection lower limits(S/N=3) were 3.90×10-5 g/L,8.09×10-5 g/L,2.96×10-5 g/L,3.98×10-5 g/L,1.46×10-4 g/L and 8.59×10-5 g/L respectively.