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Studies on Sterization and Browning of "Jinboxiang" Walnut Explants with in Vitro Propagation

Juan Wang

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Abstract

A study was carried out on explant sterization and browning of walnut explants in vitro propagation.The explants were collected from stems of Jinboxiang.The results showed that the best explant A sterization was 4 min treatments of 0.075% HgCl2 and the best explant B sterization was 4 min treatments of 0.1% HgCl2.Browning could be effecfive controled by using dark culture in prophrase,shorting shift time(took 10 d as a cycle) and adding activated carbon with 2.0 g·L-1.

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A study was carried out on explant sterization and browning of walnut explants in vitro propagation.The explants were collected from stems of Jinboxiang.The results showed that the best explant A sterization was 4 min treatments of 0.075% HgCl2 and the best explant B sterization was 4 min treatments of 0.1% HgCl2.Browning could be effecfive controled by using dark culture in prophrase,shorting shift time(took 10 d as a cycle) and adding activated carbon with 2.0 g·L-1.

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Available abstract

A study was carried out on explant sterization and browning of walnut explants in vitro propagation.The explants were collected from stems of Jinboxiang.The results showed that the best explant A sterization was 4 min treatments of 0.075% HgCl2 and the best explant B sterization was 4 min treatments of 0.1% HgCl2.Browning could be effecfive controled by using dark culture in prophrase,shorting shift time(took 10 d as a cycle) and adding activated carbon with 2.0 g·L-1.

Key concepts: Explant culture, Browning, In vitro, Botany, Horticulture, Chemistry, Biology, Biochemistry

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