2006•Jilin Nongye Daxue xuebaoRequires access
Identification of Bacillus cereus by Gyrase B gene with PCR Method
Zhenguo Wang, Heping Liu, Jinhua Liu, Yang Cai, Xiao ChengRui
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Abstract
Bacillus cereus is an important species of bacillus genus.It has close relationship in taxonomy with other Bacillus.Commonly,it is difficult to differentiate it from other Bacillus species.In this study,a PCR method for rapid and specific detection of Bacillus cereus based on the gyr B gene sequences is developed.The result shows that amplification gyr B gene by the PCR method allows faster detection of Bacillus cereus,its detection limit is 9 cfu/mL.This method can be used in healthy food control and Bacillus cereus identification.