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2009•Chinese Journal of Health Laboratory TechnologyRequires access

Detection of Bacillus cereus by denaturing high-performance liquid chromatography in food

Jijuan Cao

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Abstract

Objective:To use PCR and denaturing high-performance liquid chromatography to identify Bacillus cereus rapidly.Methods:Primers for specific PCR amplification were designed based on the published sequence of the gyrB gene of Bacillus cereus.Then its specificity,sensitivity and stability were examined.Results:Using this method to detect Bacillus cereus and other Bacteriaes of Pseudomonas,only Bacillus cereus can be detected,and as little as 10 cfu/ml of Bacillus cereus can be detected.Conclusion:A PCR-DHPLC assay amplification targeting at the gyrB gene of bacillus cereus is a rapid,specific,sensitive and relatively simple method for the identification of bacillus cereus.

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What this paper is about

Objective:To use PCR and denaturing high-performance liquid chromatography to identify Bacillus cereus rapidly.Methods:Primers for specific PCR amplification were designed based on the published sequence of the gyrB gene of Bacillus cereus.Then its specificity,sensitivity and stability were examined.Results:Using this method to detect Bacillus cereus and other Bacteriaes of Pseudomonas,only Bacillus cereus can be detected,and as little as 10 cfu/ml of Bacillus cereus can be detected.Conclusion:A PCR-DHPLC assay amplification targeting at the gyrB gene of bacillus cereus is a rapid,specific,sensitive and relatively simple method for the identification of bacillus cereus.

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Available abstract

Objective:To use PCR and denaturing high-performance liquid chromatography to identify Bacillus cereus rapidly.Methods:Primers for specific PCR amplification were designed based on the published sequence of the gyrB gene of Bacillus cereus.Then its specificity,sensitivity and stability were examined.Results:Using this method to detect Bacillus cereus and other Bacteriaes of Pseudomonas,only Bacillus cereus can be detected,and as little as 10 cfu/ml of Bacillus cereus can be detected.Conclusion:A PCR-DHPLC assay amplification targeting at the gyrB gene of bacillus cereus is a rapid,specific,sensitive and relatively simple method for the identification of bacillus cereus.

Key concepts: Bacillus cereus, Cereus, Biology, Bacillus (shape), Microbiology, Bacillaceae, Chromatography, Chemistry

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