Analysis of common causes of unsuccessful orthotopic liver transplantation in rats and their solution strategy

Objective:To analyse the common causes of unsuccessful orthotopic liver transplantation in rats,and provide an appropriate treatment strategy.Methods:Over a period of 10 months,orthotopic liver transplantation model was performed by modified Kamada two-cu technique in 150 rats.Completion of a set of 50 consecutive cases was regarded as an experimental stage,so the whole experimental course was partitioned into three stages:early,middle and late stage.The causes of transplantation failure in each stage were analyzed separately and summarized.Results:In the early stage,the major causes leading to failure of rat orthotopic liver transplantation were pneumothorax(5.8%),intraoperative bleeding(10.6%),and exended anhepatic phase(50.4%),while those in the middle and late stage were failed anastomosis of suprahepatic inferior vena cava(21.0%),extended anhepatic phase(20.6%),failed anastomosis of portal vein and infrahepatic inferior vena cava(17.6%),and failed bile duct reconstruction(5.6%).Conclusion:There are many causes of unsuccessful orthotopic liver transplantation in rats,among which,the anhepatic phase is the most critical factor a ecting the results.Timely identication of the causes of unsuccessful transplantation and nding the appropriate solutions are helpful to the novice operators.