Optimizing extraction process of polysaccharides from millet by uniform design

To optimize the technology for extracting polysaccharides from millet,the effects of particle or powder,liquid to material ratio,extraction time,extraction temperature,extraction times and stirring rate on the extraction rate were studied with single-factor experiments.Then three factors,liquid to material ratio,extraction time and extraction temperature,were selected for a uniform design experiment with twelve levels.The statistic analysis was made with DPS and the optimal combination was obtained.Temperature show notable effects on the yield of polysaccharides,liquid to material ratio and extraction time have no notable effects on the yield.The established optimum technical conditions are liquid to solid ratio 21.7 ∶ 1,extraction time 2.19 h,extraction temperature 72.3 ℃,the yield of polysaccharides is 7.90 mg/g.