Study on effects of naproxen on the proliferation and apoptosis of human cervical cancer Hela cells in vitro

Objective To investigate the effect of naproxen on proliferation and apoptosis of human cervical cancer Hela cells in vitro. Methods Hela cells were treated with different concentration of naproxen (1 μg/mL; 10 μg/mL; 100 μg/mL; 300 μg/mL; 500 μg/mL), at different times (6,12,24,48 h). Cell viability was determined by MTT assay; Cell morphology was examined by fluorescent microscope, in which cells were treated with 500 μg/mL naproxen for 48 h. Cell apoptosis was analysed by flow cytometry with Annexin V-FITC/PI staining. Results 1.MTT assay indicated that different concentration of naproxen can inhibit the proliferation of Hela cells (P 0.01) 2. In cell morphology assay, condensed cell nucleus andapoptotic-body could be observed. 3. Apoptosis rate of Hela cells were significantly increased after treated by naproxen. Conclusion Naproxen can inhibit the proliferation of cervical cancer Hela cells and induce apoptosis in vitro in a dose-dependent and time-dependent manner.