Comparison on accumulation of flavonoids in loose and compact callus suspension cell culture of Tetrastigma hemsleyanum

Objective To analyze and compare the cell growth and accumulation of its secondary metabolites,flavonoids,in loose and compact calluses suspension cell culture of Tetrastigma hemsleyanum.Methods The calluses induced from the leaves of T.hemsleyanum were subcultured in different culture media,to screen loose and compact calluses,then established two suspension cell systems.The time courses of cell growth and yields of flavonoids were studied.Results Suitable culture period of loose callus suspension cell was 8 d,however,the suitable culture period of compact callus suspension cell was 20 d.The highest multiple proliferation was 3.68,however,the highest multiple proliferation of compact callus suspension cell was 2.65.The highest content of flavonoids was 11.7 mg/g,increased by 34.5%,compact callus suspension cell was 40.9 mg/g,increased by 212.2%,loose callus suspension cell had a high level of browning and varietal vitality decline after 4-5 subcultures.There was no significant changes in the subculture of the multicell suspension cultures.Conclusion Comparing with loose callus,the compact callus suspension cell shows a remarkable high content of flavonoids,and is preferable for subculture.