Influencing Factors to Potato Transformation Mediated by Agrobacterium

In this paper,the effects of different explants,the durations of pre-culture and co-culture on transformation frequency mediated by Agrobacterium were studied.The results showed that calli could be induced from both leaves and stems of potatoes and that optimized system was pre-cultured of explants for 2 days before transformation,infecting explants by Agrobacterium with 0.5 OD600 for 10 min,co-culture of explants and Agrobacterium for 3 days.A ribozyme gene targeting against two sites on negative strand of potato spindle tuber viroid(PSTVd) was transformed into potato by this optimized system.