Optimization for supercritical CO_2 extraction of Perilla frutescens oil by response surface methodology and component analysis

Supercritical CO_2 extraction to determine the optimum conditions Perilla frutescens oil.Using Box-Behnken response surface design was used to Perilla frutescens oil extraction are evaluated and optimal screening supercritical fluid extraction to DesignExpert software for data compared with actual results to be merged.Supercritical CO_2 extraction to determine optimum conditions Perilla frutescens oil: extraction temperature 60℃,extraction pressure 32.5 MPa,extraction time 180 min,in this condition, Perilla frutescens oil extraction rate of 39.88%,with the calculated deviation is less than 10%.Confirmation of the four elements of fatty acids by GOMS analyzing: Octadecadienoic acid(11.889%),Octadecatrienoic acid(79.088%),Hexadecanoic acid (6.813%),Octadecanoic acid(2.290%).The Box-Behnken response surface methodology optimization of Perilla frutescens physical and chemical properties of supercritical extraction conditions and stability,the theoretical value of the index and can better fit, numerical model,reasonable and effective.Ingredients of unsaturated fatty acid in Perilla frutescens oil are high,so it can be developed and utilized scale.