Determination of α-Linolenic Acid,Linoleic Acid and Oleic Acid in Oviductus Ranae by HPLC

Objective: To establish a HPLC method for the determination of α-linolenic acid,linoleic acid and oleic acid in Oviductus Ranae.Method: Alltima C18(4.6 mm × 250 mm,5 μm) column was used with mobile phase of acetonitrile-0.1% phosphoric acid(80 ∶ 20);the flow rate was 1 mL.min-1;the column temperature was kept at 30 ℃;the detection wavelength was set at 203 nm.Result: The linear ranges of αlinolenic acid,linoleic acid and oleic acid were 4.755-152.16 mg.L-1(r =1),15.362-491.59 mg.L-1(r = 0.999 8) and 47.275-1512.8 mg.L-1(r = 0.999 9) respectively.The average recoveries were 95.8% with RSD 1.79% for α-linolenic acid,98.6% with RSD 1.32% for linoleic acid,99.3% with RSD 1.59% for oleic acid.Conclusion: The method is simple,accurate,convenient,specific and repeatable,which can be used to control the quality of α-linolenic acid,linoleic acid and oleic acid in Oviductus Ranae.