Mutagenesis of Alkaline Cellulase High-producing Bacillus Strain sp.CY1-3
Yong Cai
Abstract
Yong Cai
Abstract
A strain of Bacillus sp.CY1-3 which could produce endo-1,4-β-glucanase was mutagenized by UV and NTG,gave a cellulase yield of 53.86 U/mL which was 4 times as that of original strain.Results showed that the optimum UV irradiation time was four minutes and the best NTG concentration was 0.06%.
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A strain of Bacillus sp.CY1-3 which could produce endo-1,4-β-glucanase was mutagenized by UV and NTG,gave a cellulase yield of 53.86 U/mL which was 4 times as that of original strain.Results showed that the optimum UV irradiation time was four minutes and the best NTG concentration was 0.06%.
Key concepts: Cellulase, Strain (injury), Mutagenesis, Food science, Chemistry, Bacillus (shape), Microbiology, Enzyme