Mutation and Screening for Cellulase Production Strain

[Objective]The research aimed to improve enzyme activity of cellulase production strain.[Method] A strain named Y-07 was isolated from rotten straw soil nearby the suburb of Fushun with culture medium containing the carboxymethylcellulose sodium and Congo Red in accordance with its larger size of ratio of hydrolysis ring to colony diameter.With the strain of Y-07 as starting strain,the strain of Y-07 was mutagenized by ultraviolet radiation and nitrous acid mutagenesis under the most appropriate amount of mutation.[Result] After three rounds of secondary screening,the strain of U10 was achieved with the biggest cellulase activity 2.5 IU/ml which was 3.4 times of Y-07 strain.[Conclusion]The method provided the basis for further improvements of enzyme activity of cellulase production strain.