2003Indian Journal of Genetics and Plant Breeding (The)Requires access

Evaluation of genetic diversity in Indian tetraploid and diploid cotton (Gossypium spp.) by morphological characteristics and RAPDs

Mosareza Vafaie-Tabar, S. Chandrashekaran, Rupinder Pal Singh, Maneesha Rana

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Abstract

Genetic diversity was evaluated among 7 diploid (G. arboreum) and 15 tetraploid (G. hirsutum) cotton cultivars using Random Amplified Polymorphic DNA (RAPD) and morphological markers. RAPD markers were efficient and detected 88% polymorphism. The diploid and tetraploid cultivars could be divided into separate clusters at 30% similarity with RAPD and 20% similarity with morphometric markers. Classification of the cultivars based on the two markers showed a high degree of agreement with a correlation of (+) 0.90.Both markers revealed higher diversity among diploids than among tetraploids.

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Genetic diversity was evaluated among 7 diploid (G. arboreum) and 15 tetraploid (G. hirsutum) cotton cultivars using Random Amplified Polymorphic DNA (RAPD) and morphological markers. RAPD markers were efficient and detected 88% polymorphism. The diploid and tetraploid cultivars could be divided into separate clusters at 30% similarity with RAPD and 20% similarity with morphometric markers. Classification of the cultivars based on the two markers showed a high degree of agreement with a correlation of (+) 0.90.Both markers revealed higher diversity among diploids than among tetraploids.

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Available abstract

Genetic diversity was evaluated among 7 diploid (G. arboreum) and 15 tetraploid (G. hirsutum) cotton cultivars using Random Amplified Polymorphic DNA (RAPD) and morphological markers. RAPD markers were efficient and detected 88% polymorphism. The diploid and tetraploid cultivars could be divided into separate clusters at 30% similarity with RAPD and 20% similarity with morphometric markers. Classification of the cultivars based on the two markers showed a high degree of agreement with a correlation of (+) 0.90.Both markers revealed higher diversity among diploids than among tetraploids.

Key concepts: RAPD, Biology, Ploidy, Genetic diversity, Gossypium, Cultivar, Gossypium hirsutum, Genetic similarity

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