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Optimization of Extraction Conditions of Total Flavonoids from Lotus Leaves by Plackett-Burman Design and Response Surface Analysis

Yang Ji-yan, Hu Lei, Yang Xu

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Abstract

On the basis of single-factor tests, some factors affecting the extraction rate of total flavonoids from lotus leaves were firstly evaluated by Plackett-Burman design, and then the extraction conditions were optimized by response surface analysis. The results showed that the optimal extraction conditions are as follows: ethanol concentration 67%, ratio of ethanol solution to raw material 34∶1 and extraction times 3. Under the above conditions, the predicted value of extraction rate of total flavonoids is 3.19% and its experimental value is 3.17%, which indicated that the combination of Plackett-Burman design and response surface analysis can well optimize the extraction technology of total flavonoids from lotus leaves.

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What this paper is about

On the basis of single-factor tests, some factors affecting the extraction rate of total flavonoids from lotus leaves were firstly evaluated by Plackett-Burman design, and then the extraction conditions were optimized by response surface analysis. The results showed that the optimal extraction conditions are as follows: ethanol concentration 67%, ratio of ethanol solution to raw material 34∶1 and extraction times 3. Under the above conditions, the predicted value of extraction rate of total flavonoids is 3.19% and its experimental value is 3.17%, which indicated that the combination of Plackett-Burman design and response surface analysis can well optimize the extraction technology of total flavonoids from lotus leaves.

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Available abstract

On the basis of single-factor tests, some factors affecting the extraction rate of total flavonoids from lotus leaves were firstly evaluated by Plackett-Burman design, and then the extraction conditions were optimized by response surface analysis. The results showed that the optimal extraction conditions are as follows: ethanol concentration 67%, ratio of ethanol solution to raw material 34∶1 and extraction times 3. Under the above conditions, the predicted value of extraction rate of total flavonoids is 3.19% and its experimental value is 3.17%, which indicated that the combination of Plackett-Burman design and response surface analysis can well optimize the extraction technology of total flavonoids from lotus leaves.

Key concepts: Plackett–Burman design, Extraction (chemistry), Response surface methodology, Raw material, Lotus effect, Lotus, Chemistry, Chromatography

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