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Effects of anthocyanin on matrix metalloproteinase expression in human umbilical vein endothelial cell

Zhihong Tang

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Abstract

Objective To explore the effect of anthocyanin on matrix metalloproteinases (MMP) expression and activity. Methods Human umbilical vein endothelial cell were isolated and incubated with 5μg/mL rCD40L for 24h. After that,the endothelial cell were incubated with 1μM,10μM,100μM C3G and P3G for another 24h, respectively. Methods of zymography and Western blotting were used to analyze MMP-1, MMP-2, MMP-9 activities and protein expression.Results Anthocyanin C3G and P3G inhibited MMP-1, MMP-2 and MMP-9 protein expression and their activities significantly.Conclusion Anthocyanin C3G and P3G inhibited MMP-1,MMP-2,MMP-9 protein expression and their activities.

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What this paper is about

Objective To explore the effect of anthocyanin on matrix metalloproteinases (MMP) expression and activity. Methods Human umbilical vein endothelial cell were isolated and incubated with 5μg/mL rCD40L for 24h. After that,the endothelial cell were incubated with 1μM,10μM,100μM C3G and P3G for another 24h, respectively. Methods of zymography and Western blotting were used to analyze MMP-1, MMP-2, MMP-9 activities and protein expression.Results Anthocyanin C3G and P3G inhibited MMP-1, MMP-2 and MMP-9 protein expression and their activities significantly.Conclusion Anthocyanin C3G and P3G inhibited MMP-1,MMP-2,MMP-9 protein expression and their activities.

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Available abstract

Objective To explore the effect of anthocyanin on matrix metalloproteinases (MMP) expression and activity. Methods Human umbilical vein endothelial cell were isolated and incubated with 5μg/mL rCD40L for 24h. After that,the endothelial cell were incubated with 1μM,10μM,100μM C3G and P3G for another 24h, respectively. Methods of zymography and Western blotting were used to analyze MMP-1, MMP-2, MMP-9 activities and protein expression.Results Anthocyanin C3G and P3G inhibited MMP-1, MMP-2 and MMP-9 protein expression and their activities significantly.Conclusion Anthocyanin C3G and P3G inhibited MMP-1,MMP-2,MMP-9 protein expression and their activities.

Key concepts: Umbilical vein, Matrix metalloproteinase, Zymography, Anthocyanin, Human umbilical vein endothelial cell, Blot, Chemistry, Matrix (chemical analysis)

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