2006•Tianjin yiyaoRequires access
Effects of Energy Inhibitor Carbonyl Cyanaide m-Chlorophenylhydrazone (CCCP) on Accumulation of Ciprofloxacin in Enterobater Cloacae Clinical Isolates
Si Jin
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Abstract
Objective: To investigate the effects of clinical isolates of Enterobacter Cloacae on accumulation of ciprofloxacin and the effects of carbonyl cyanide m-chlorophenylhydrazone(CCCP) on uptake of ciprofloxacin in E.cloacae isolates. To explore the effects of initiative pump mechanism on drug resistance of quinolones. Methods: The accumulation of ciprofloxacin was determined by using a modification of fluorometric assay, and the intracellular concentration of ciprofloxacin was compared before and after the use of CCCP. Results: The accumulation of ciprofloxacin for all detected isolates of E.cloacae reached to a stable concentration within 5 min, and the intracellular stable concentration of ciprofloxacin was lower in quinolone-resistant isolates than that in quinolone-susceptible isolates. The accumulation of ciprofloxacin in resistant isolates increased after incubated with CCCP. Conclusion: Energy-dependent active efflux may play a certain role in ciprofloxacin resistance in E.cloacae by decreasing the accumulation of ciprofloxacin.