Improved techniques for murine model of cardiac transplantation

Objective To establish a murine model of cardiac transplantation for basic research in this field.Methods The aorta of the graft was anastemosed with recipient's abdominal aorta with a end_to_side continuous suture method,while the pulmonary artery of the graft was anastomosed with recipient's inferior vena cava in the similar way.Results The success rate for mice cardiac transplantation was 90.0%.The operation time for donor was (7.0±1.0)min and for recipient was (52.5±10.5)min.The arterial and venous anastomosis took (10.0±2.0) min and (9.0±2.0) min respectively.The length of large vessels used for anastomosis was (6.5±1.0)mm,while the diameter of IVC and aorta was (2.4±0.2) mm and (1.0±0.1)mm respectively.Conclusion The improved method murine cardiac transplantation is reliable one,which can be used in basic research of transplant immunology.