Protective Effects of Combination of Ligustrazine and Ferulic Acid on Myocardial Injury Induced by Ischemia and Reperfusion in Rats

Objective:To explore the protective effects of ligustrazine,ferulic acid,baicalin and combination on myocardial injury induced by ischemia and reperfusion in rats.Methods:Sixty-four male SD rats with weight of(220±10) g were randomly divided into four groups which included normal group,false operation group,model group and ligustrazine and ferulic acid group.Myocardial ischemia reperfusion injury model was established in the last two groups.Six hours after establishing the model,the serum creatine phosphokinase(CK) ,lactate dehydrogenase(LDH) ,the myocardial malondialdehyed(MDA) ,and the myocardial super oxide dismutase(SOD) activity were measured.The expression of intercellular adhesion molecule(ICAM-1) ,P-selection and E-selection were detected by Real Time PCR.Results:Ligustrazine and ferulic acid group played an important role in reducing serum CK,but the effect was not significant on serum LDH and SOD and MDA in myocardial injury.The expression of E-selection and P-selection could be decreased markedly by ligustrazine and ferulic acid.Conclusion:Combination of ligustrazine and ferulic acid exerts a significant protective effect on myocardial ischemia reperfusion injury.