The Effect of CTP on Neuronal Apoptosis and Cognitive Function after Serve Traumatic BrainInjury in Rats

Objective:To investigate the effect of CTP on neuronal apoptosis and cognitive function after severe traumatic brain injury(TBI)in rats.Methods:The model of severe closed TBI was established according to the method created by Marmarou.Two hundred and twenty-eight Wistar rats were randomly divided into TBI group,CTP treating group,and fake operation group,and each of them was divided into3,6,12,24,48,72,168,and336hour groups.Twelve rats were taken as normal control.The CTP treating group after injury was treated with CTP[30mg/(kg·d)]in peritoneum.TBI group,the fake operation group,and control group were treated with Saline.All rats were killed at each time point,and apoptosis in situ were detected.Another48rats were randomly divided into brain injury group,CTP treating group,fake operation group,and normal group.The cognitive dysfunction was evaluated using the Morris water maze(MWM).Results:CTP could decrease the apex of apoptosis-positive cells in hippocampus and shorten the delitescence of Morris water maze test.Conclusions:CTP may effectively suppress apoptosis of nerve cells after TBI and improve cognitive function.