Effect of laparoscopic surgery on human phagocyte & erythrocyte immune activities of patients with benign gynecologic disorders

[Objective] In order to provide further evidences for minimum incisions induced by the laparoscopic procedures. This study was designed to evaluate the effect of laparoscopic surgery on the host immune response in patients with benign gynecologic disorders required to operation and finds its advantages by comparing with open surgery. [Methods] Sixty patients with benign gynecologic disorders required to surgery were assigned to laparoscopic surgery (LS, n =32) and open surgery (OS, n =28). White blood cell count and its kinds, serum concentrations of IL-6 and TNF-α and erythrocyte immune function were determined at 24 h preoperatively, 24 h and 72 h postoperatively, respectively. [Results] In both groups, these parameters including WBC count [OS group:(5.22±1.31) vs (10.33±2.37), P 0.05, LS group: (5.66±1.48) vs (7.81±1.75), P 0.05], neutrophil proportion [OS group: (56.38±9.73) vs (81.76±5.79), P 0.05, LS group: (56.64±9.72) vs (75.38±10.79),P 0.05], the values of IL-6 [OS group: (3.14±0.98) vs (26.72±15.59), P 0.05; LS group: (3.29±1.25) vs (17.94±10.93), P 0.05] and TNF-α [OS group: (54.83±17.99) vs (80.70±15.32), P 0.05; LS group: (54.39±11.19) vs (76.16±19.71), P 0.05] significantly increased at postoperative 24 h compared with preoperative level and decreased at postoperative 72 h. But increasing degree of WBC [(7.81±1.75) vs (10.33±2.37), P24 h 0.05; (5.88±1.28) vs (7.12±1.32), P72h 0.05] and IL-6 [(17.94±10.93) vs (26.72±15.59), P 0.05] was lower in laparoscopy group than that in laparotomy group significantly at postoperative 24 h. The values of CD35 expression and C3bRR hadn't obvious change in patients who underwent laparoscopic approach before and after surgery (P 0.05). The values of C3bRR went down dramatically at postoperative 24 h [(22.05±2.11) vs (16.10±1.31), P 0.05], and returned to a lower level at postoperative 72 h compared with preoperative 24 h [(22.05±2.11) vs (16.15±2.93), P 0.05]. The values of ICR hadn′t display obvious change between groups and before or after operation (P 0.05). [Conclusion] Immune suppression on mononuclear phagocytic system of the body to trauma in laparoscopic surgery was lighter than that in open surgery. No significant effect on RBC immune function of host body with benign gynecologic disorders in laparoscopic surgery was found.