Effects of enteral nutrition and parenteral nutrition on bowel barrier function in rats with obstructive jaundice

Objective To compare the effects of enteral nutrition(EN) and parenteral nutrition(PN) on bowel mucosal barrier function in rats with obstructive jaundice.Methods By common biliary duct ligating,the obstructive jaundice rat models were set up.Sixty SD rats were randomly divided into group A:sham operation(SHAM),group B:common biliary duct ligating(CBDL),group C:CBDL+TPN,group D:CBDL+EN,group E:CBDL+ freedom drinking water with antibiotic,12 rats in each group.After the rats were bred for 1 week,the caval vein blood was got and the serum was separated to wait for the measuring of endotoxin.The mesenteric lymph node,liver and spleen were obtained,cultivated,observed and the condition of bacterium growth were recorded.The small intestine was gained to make microtome section and mucous membrane of small intestine morphology,trophonema altitude,thickness and crypt shade were observed under light microscope.Results Compared with group A,jejunal mucous intestinal membrane depths in groups B and D were shallow(P0.05),rat jejunal mucous intestinal membrane in group C and E became thinner and atrophy,and had shortened villus and shallower cave.There was no lymphnode mesenterici bacteria transposal in group A,the lymph node mesenterici bacteria transposal rates in groups B,C,D and E were higher than that in group A(P0.01 or P0.05).The contents of endotoxin in group B,C,D and E were higher than that in group A(P0.01 or P0.05),it was lower in group E than those in groups B,C and D(P0.05).Conclusion ①When intestinal barrier is injuried,bacteria transposal and endotoxemia exist in obstructive jaundice;②Both EN and standard PN can not maintain intestinal membrane barrier and hold up the occurrence of intestinal bacteria transposal in obstructive jaundice rats,but the effect of EN is better than PN;③Intestinal tract antibiotics can step down incidence rate of endotoxemia and profit to hold up the occurrence of intestinal bacteria transposal.