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2013•Chinese Journal of Spectroscopy LaboratoryRequires access

Application of a Fast Protein Staining Method with CBBG-250 in SDS-PAGE

Wu Yuan

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Abstract

The dyeing conditions for a fast protein staining method,combining microwaveheating with Coomassie Brilliant Blue G-250(CBB G-250),were investigated in SDS-PAGE.The whole procedure,which can be finished within 1 hour,is much faster than the conventional CBB R-250 staining protocol.No organic solvent and other acids but 3mmol/L hydrochloric acid was used in the method,which is benefical to operators and environment-friendly.Moreover,the obtained sensitivity was similar with that of the classic staining protocol.The method is applicable to the rapid analysis of protein electrophoresis in the experimental teaching and scientific research.

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What this paper is about

The dyeing conditions for a fast protein staining method,combining microwaveheating with Coomassie Brilliant Blue G-250(CBB G-250),were investigated in SDS-PAGE.The whole procedure,which can be finished within 1 hour,is much faster than the conventional CBB R-250 staining protocol.No organic solvent and other acids but 3mmol/L hydrochloric acid was used in the method,which is benefical to operators and environment-friendly.Moreover,the obtained sensitivity was similar with that of the classic staining protocol.The method is applicable to the rapid analysis of protein electrophoresis in the experimental teaching and scientific research.

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Available abstract

The dyeing conditions for a fast protein staining method,combining microwaveheating with Coomassie Brilliant Blue G-250(CBB G-250),were investigated in SDS-PAGE.The whole procedure,which can be finished within 1 hour,is much faster than the conventional CBB R-250 staining protocol.No organic solvent and other acids but 3mmol/L hydrochloric acid was used in the method,which is benefical to operators and environment-friendly.Moreover,the obtained sensitivity was similar with that of the classic staining protocol.The method is applicable to the rapid analysis of protein electrophoresis in the experimental teaching and scientific research.

Key concepts: Coomassie Brilliant Blue, Staining, Chromatography, Dyeing, Chemistry, Hydrochloric acid, Solvent, Bradford protein assay

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