Study on the Cause and Restoration of Vitrification PLB in Tissue Culture and Rapid Propagation of Phalaenopsis

Studying on the causes and the recycle effects of vitrification protocorm in manufacture of Phalaenopsis.Results showed that:it aggravates the extent of protocorm vitrification with the increase of hormone concentration and the expansion of subculture time.The vitrification rates of two culture mediums rise to 71% and 65% respectively when the concentration of hormone NAA was 5mg/L,hormone BA was 10 mg/L and subculture the ninth generation.The reproduction ability and plant regeneration rate was lower distinctly than formal protocorm.The phenomenon of vitrification could restore significantly after culture 2 or 3 generations in the culture medium without hormone.The differentiation ability of protocorm restored and the state of plant growth was consistent with the formal protocorm.It could continue applying to the manufacture of tissue culture.