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2007•China Animal Husbandry & Veterinary MedicineRequires access

Detection of Salmonella in Chicken with Fluorescence Polymerase Chain Reaction

Lin Mei

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Abstract

In present article,there are two positive standard salmonella bacteria,two positive sample about chicken and a sample of aseptic water.This samples are detected with fluorescence polymerase chain reaction.The results showed: The method is efficient and accurate.Furthermore,it took only 30 hours for whole PCR process.It is concluded that PCR is a specific,sensitive,rapid,simple and convenient method for detection of salmonella.It may have great values for the entry-exit inspection and quarantine.

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What this paper is about

In present article,there are two positive standard salmonella bacteria,two positive sample about chicken and a sample of aseptic water.This samples are detected with fluorescence polymerase chain reaction.The results showed: The method is efficient and accurate.Furthermore,it took only 30 hours for whole PCR process.It is concluded that PCR is a specific,sensitive,rapid,simple and convenient method for detection of salmonella.It may have great values for the entry-exit inspection and quarantine.

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Available abstract

In present article,there are two positive standard salmonella bacteria,two positive sample about chicken and a sample of aseptic water.This samples are detected with fluorescence polymerase chain reaction.The results showed: The method is efficient and accurate.Furthermore,it took only 30 hours for whole PCR process.It is concluded that PCR is a specific,sensitive,rapid,simple and convenient method for detection of salmonella.It may have great values for the entry-exit inspection and quarantine.

Key concepts: Salmonella, Polymerase chain reaction, Real-time polymerase chain reaction, Aseptic processing, Bacteria, Microbiology, Chemistry, Biology

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