Establishment and application of PCR method of detection for swine Pasteurella multocida

A set of specific primers to amplify 457 bp DNA product was designed to detect swine Pasteurella multocida. The lung tissues or nasal swabs from pneumonic swine and atrophic rhinitis swine were streaked on TSA, then the bacteria clones were detected by the PCR and by the biochemical reaction after the bacteria were purified. With the mentioned-above methods, 66 strains of Pasteurella multocida were (isolated) from the different areas. The PCR result was quite coincident with that of the biochemical reaction. The detection results of 6 other swine respiratory pathogenic bacteria were negative by the set of primers. CFU 1×10~3/mL Pasteurella multocida template can be detected by the PCR.