The endoplasmic reticulun stress signaling pathways for Atorvastatin in protection of myocardium in ischemia/reperfusion injury model rats

Objective To observe the effects of Atorvastatin on the expression of GRP78 and GADD153 proteins in rats with schemia/reperfusion injury,so as to explore the the endoplasmic reticulun stress signaling pathways for Atorvastatin in protection of myocardium.Methods 54 SD rats were randomly divided into the Atorvastatin intervention group(n=24): the rats were infused orally with Atorvastatin,20 mg/(kg·d);the model control group(n=24): the rats were infused orally with 0.9% sodium chloride solution,2ml/d;the pseudo-operation group(n=6): the rats were infused orally with 0.9% sodium chloride solution,2ml/d.The schemia/reperfusion injury rats models were produced.The TUNEL kit was used to detect myocardial cells apotosis.The immunohistochemistry technique was used to detect GRP78 and GADD153 proteins.Results The Atorvastatin intervention group yielded less myocardial cells apoptosis at adjacent time points,compared to the model control group(P0.01).At corresponding time point,the elevated GRP78 proteins were obtained in Atorvastatin intervention group,compared to the model control group(P0.05),while the levels of GADD153 protein decreased significantly,compared to the the model control group(P0.01 or P0.05). Conclusion Via endoplasmic reticulun stress signaling pathways may be one of the mechanism of Atorvastatin in protection of myocardium in ischemia/reperfusion injury.